descriptionPublicationkeyboard_double_arrow_right Article , Conference object , Other literature type 01 Dec 2018 English Publisher:Elsevier BVJournal:Biotechnology Advances, volume 36, pages 2,150-2,164 (issn: 0734-9750,

Authors: Larroudé, Macarena; Rossignol, Tristan; Nicaud, Jean-Marc; Ledesma Amaro, Rodrigo;

doi: 10.1016/j.biotechadv.2018.10.004

pmid: 30315870

pmc: PMC6261845

handle: 10044/1/65509

Synthetic biology tools for engineering Yarrowia lipolytica

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Abstract

The non-conventional oleaginous yeast Yarrowia lipolytica shows great industrial promise. It naturally produces certain compounds of interest but can also artificially generate non-native metabolites, thanks to an engineering process made possible by the significant expansion of a dedicated genetic toolbox. In this review, we present recently developed synthetic biology tools that facilitate the manipulation of Y. lipolytica, including 1) DNA assembly techniques, 2) DNA parts for constructing expression cassettes, 3) genome-editing techniques, and 4) computational tools.

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Keywords

Yarrowia lipolytica, PROTEIN EXPRESSION, [SDV]Life Sciences [q-bio], OMEGA-3 EICOSAPENTAENOIC ACID, Yarrowia, Industrial biotechnology, HOMOLOGOUS RECOMBINATION, 09 Engineering, Article, RAW GLYCEROL, 10 Technology, DNA assembly, Synthetic biology, GENE-EXPRESSION, ALPHA-KETOGLUTARATE, Gene Editing, Science & Technology, Golden Gate, CITRIC-ACID, 06 Biological Sciences, 540, [SDV] Life Sciences [q-bio], Genome scale metabolic models, Biotechnology & Applied Microbiology, Metabolic Engineering, HETEROLOGOUS PROTEINS, Synthetic Biology, CRISPR-Cas9, CRISPR-Cas Systems, LIPID-ACCUMULATION, Life Sciences & Biomedicine, Metabolic engineering, HANSENULA-POLYMORPHA, Genome editing, Biotechnology

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