One hundred and eleven rice seeds samples of 79 different rice varieties were collected from five different agricultural research farms; Myaungmya, Letpadan, Naungmon, Yezin and Kyaukse in monsoon season to determine the infestation of Aphelenchoides besseyi. The observation showed that A. besseyi was found in rice seeds samples collected from five agricultural research farms. Fifty three out of 111 rice seeds samples were infested with A. besseyi ranged from 1 to 424 nematodes 100 seeds-1. Among study sites, 45% rice varieties in Myaungmya, 10% in Letpadan and 14% in Naungmon were infested with economic threshold level of 30 nematodes 100 seeds-1. Four inoculation methods of introducing nematode suspension; below the leaf sheath (T1), into four holes of soil (T2), spraying nematode suspension to the plant (T3) and dipping the rice seedlings into nematode suspension (T4) were studied to evaluate pathogenicity of A. besseyi in rice. Results showed that among tested inoculation methods, higher final nematode population was found in three methods; T4, T1 and T2 with number of 103, 59 and 47 nematodes 100 seeds-1, respectively than that of one nematode 100 seeds-1 in T3. The reactions of fifteen rice varieties which were widely cultivated throughout Myanmar to A. besseyi were assessed with inoculum level of 500 nematodes plant-1 by artificial inoculation of introducing nematode suspension into four holes of soil around the plant. Among them, Paw Hsan Yin, Sin A Kari-3 and Yadana Toe showed moderately resistant reaction while Aye Yar Min, Hnangar, Thee Dat Yin, and Hmawbi-2 were moderately susceptible and Manawthukha, Sin Thu Kha, Shwe War Tun, Sin Thwe Latt, Kyaw Zay Ya, Shwe Bo Paw Hsan, Shwethwe Yin and Shwe Yin Aye were highly susceptible to A. besseyi. Effect of different control measures such as soaking infested rice seeds in brine solution (20% NaCl) (T2), seed treated with hot water at 55°C for 30 minutes (T3), application of carbofuran (Furadun 3G with 3% Carbofuran w/w) to soil (T4), combination of seed treated with hot water at 55°C for 30 minutes and brine solution (T5) and combination of seed treated with hot water at 55°C for 30 minutes, brine solution and application of carbofuran to the soil (T6) were conducted to evaluate the effective management of white tip disease in the screen house by using the infested Lone Pu variety. The reduction percent of nematode population were found as 100% in T4, 99.9% in T6, 99.8% in T3 and 99.5% in T5 and these treatments seemed to have good effect in yield and yield components. Accordingly, it could be suggested that nematode infested seeds treated with hot water treatment alone (T3) can be used to minimize the infestation of A. besseyi and reduce the loss of grain yield.