The crayfish subgenus Procericambarus (genus Orconectes) found in the Eastern Highlands of the United States represents a rich assemblage of stream-dwelling macroinvertebrates. While the taxonomic status and distribution of its members were clarified in a previous study, newly diagnosed characters and newly collected specimens warranted a revision to previous hypotheses of Procericambarus membership in the southeastern United States. Using morphological characters, we describe herein a new species of crayfish occurring in the Tennessee River drainage of northern Alabama and southwestern Tennessee. We also provide a revised key for the identification of members of O. juvenilis Species Complex.