It is not easy to design an appropriate indicator of network collaboration that is relevant and comparable to all regions of the Russian Federation. This is a challenge because the creation of high-tech start-ups requires the cooperation of various and heterogeneous stakeholders: inventor, entrepreneur, investor, and the university. Accordingly, the purpose of this paper is to test the following hypothesis: the greater the number of viable spin-offs in the region, the higher the level of innovative activity of the region. The object of analysis is Russian spin-offs, which were created during the period from August 2009 to May 2014. Using their founders’ profiles, I obtained the university affiliation of these companies. I also gathered information about their industry affiliation, size, turnover, regional (country) identity, etc. Using this data, I developed a number of indicators characterizing the intensity of interaction between universities and companies. I show that there is a positive link between innovation activity and performance indicators describing the intensity of interaction between universities and companies at the regional level. In closing, I offer a few comments about the possibility of using the results of this research in the analysis of applied problems related to innovation policy and the development of ratings for the innovative activity of regions.