A nanoparticle originating from natural products used as a drug delivery is considered as one of the indispensable issues in the pharmaceutical field. Increasing the bioavailability of a drug and prolonging the effect of the drug are important. Tinidazole is an antifungal agent that has absorption interfered by food. This study reported the ability ofLeuconostoc mesenteroidesVTCC B-871 in producing nanosized minicells used as drug delivery for tinidazole to improve the passage to the eaten mouse intestinal membrane. By using a scanning electron microscope and a transmission electron microscope, the morphology of the minicell loading drug was observed. The spherical shape and size (400 nm) of minicells did not change over time when kept in buffered saline gelatin and packaged with tinidazole. Based on Box-Behnken design, the optimal conditions were selected for actual encapsulation. Minicells could encapsulate tinidazole approximately to 90% which was determined by high-performance liquid chromatography analysis. The maximal concentration of tinidazole released from minicells was 70% at pH 3.4 and 55% at pH 7.2, respectively. The absorption ability of tinidazole packaging minicells was quantified in mice. Tinidazole loading minicells could be absorbed faster than tinidazole alone in fed mice via oral administration. The study assessed that the absorption of water-insoluble tinidazole could be improved byLeuconostocminicells without inhibition by food effects.