
pmid: 22634702
An aerobic actinomycete, designated SCSIO 01299T, was isolated from a deep-sea sediment collected from the northern South China Sea at a depth of 3258 m. The isolate was found to be a natural producer of the synthesized antitumour agent deoxynyboquinone and its three new derivatives, pseudonocardians A, B and C. A blast search based on almost-complete 16S rRNA gene sequences showed that strain SCSIO 01299T had high sequence similarities with members of the genus Pseudonocardia . The 16S rRNA gene sequence phylogenetic tree revealed that strain SCSIO 01299T was a member of the genus Pseudonocardia . Phenotypic analysis, chemotaxonomy and DNA–DNA relatedness could readily distinguish the isolate from established members in this genus. It was concluded that strain SCSIO 01299T represents a novel species, for which the name Pseudonocardia antitumoralis sp. nov. is proposed. The type strain is SCSIO 01299T ( = DSM 45322T = CCTCC M 2011255T).
DNA, Bacterial, China, Geologic Sediments, Fatty Acids, Molecular Sequence Data, Quinones, Nucleic Acid Hybridization, Vitamin K 2, Sequence Analysis, DNA, Bacterial Typing Techniques, RNA, Ribosomal, 16S, Actinomycetales, Seawater, Water Microbiology, Phylogeny
DNA, Bacterial, China, Geologic Sediments, Fatty Acids, Molecular Sequence Data, Quinones, Nucleic Acid Hybridization, Vitamin K 2, Sequence Analysis, DNA, Bacterial Typing Techniques, RNA, Ribosomal, 16S, Actinomycetales, Seawater, Water Microbiology, Phylogeny
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