A unicum of tractate Niddah, printed in Prague in the first decade of the seventeenth century, sheds light on a previously unknown attempt by Rabbi Judah Loew to implement his pedagogical theories. This chapter describes the Niddah, to attempt to identify its printer, and to determine the circumstances of its publication. The physical characteristics of the treatise can be simply stated. The fragment consists of nine leaves, a title page and eight text leaves, and measures about 19 cm. Based on the information on the title page, we can place the date of printing between 1597, the year Maharal became Chief Rabbi of Prague. Niddah is not a tractate for initiating young students into the intricacies of the Talmud and yet the format of this edition is not one to appeal to older students of Talmud.Keywords: Maharal; Prague; Rabbi Judah Loew; Talmud; tractate Niddah