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AbstractUnlike nuclear nucleolin, surface-expressed and cytoplasmic nucleolin exhibit Tn antigen. Here, we show localization-dependent differences in the glycosylation and proteolysis patterns of nucleolin. Our results provide evidence for different paths of nucleolin proteolysis in the nucleus, in the cytoplasm, and on the cell surface. We found that full-length nucleolin and some proteolytic fragments coexist within live cells and are not solely the result of the preparation procedure. Extranuclear nucleolin undergoes N- and O-glycosylation, and unlike cytoplasmic nucleolin, membrane-associated nucleolin is not fucosylated. Here, we show for the first time that nucleolin and endogenous galectin-3 exist in the same complexes in the nucleolus, the cytoplasm, and on the cell surface of melanoma cells. Assessments of the interaction of nucleolin with galectin-3 revealed nucleolar co-localization in interphase, suggesting that galectin-3 may be involved in DNA organization and ribosome biogenesis.
Cytoplasm, Glycosylation, Galectin 3, Blotting, Western, confocal microscopy, Cell Fractionation, Mass Spectrometry, Cell Line, Tumor, Lectins, galectin-3, melanoma, Humans, glycosylation of nucleolin, Melanoma, mass spectrometry, Cell Nucleus, Nucleolin, Microscopy, Confocal, Cell Membrane, RNA-Binding Proteins, lectin assay, co-immunoprecipitation, Phosphoproteins, Proteolysis, Cell Nucleolus, Research Article, Protein Binding
Cytoplasm, Glycosylation, Galectin 3, Blotting, Western, confocal microscopy, Cell Fractionation, Mass Spectrometry, Cell Line, Tumor, Lectins, galectin-3, melanoma, Humans, glycosylation of nucleolin, Melanoma, mass spectrometry, Cell Nucleus, Nucleolin, Microscopy, Confocal, Cell Membrane, RNA-Binding Proteins, lectin assay, co-immunoprecipitation, Phosphoproteins, Proteolysis, Cell Nucleolus, Research Article, Protein Binding
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically). | 11 | |
popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network. | Top 10% | |
influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically). | Average | |
impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network. | Average |