The plant energy-dissipating mitochondrial systems: depicting the genomic structure and the expression profiles of the gene families of uncoupling protein and alternative oxidase in monocots and dicots
Copyright policy )The plant energy-dissipating mitochondrial systems: depicting the genomic structure and the expression profiles of the gene families of uncoupling protein and alternative oxidase in monocots and dicots
The simultaneous existence of alternative oxidases and uncoupling proteins in plants has raised the question as to why plants need two energy-dissipating systems with apparently similar physiological functions. A probably complete plant uncoupling protein gene family is described and the expression profiles of this family compared with the multigene family of alternative oxidases in Arabidopsis thaliana and sugarcane (Saccharum sp.) employed as dicot and monocot models, respectively. In total, six uncoupling protein genes, AtPUMP1-6, were recognized within the Arabidopsis genome and five (SsPUMP1-5) in a sugarcane EST database. The recombinant AtPUMP5 protein displayed similar biochemical properties as AtPUMP1. Sugarcane possessed four Arabidopsis AOx1-type orthologues (SsAOx1a-1d); no sugarcane orthologue corresponding to Arabidopsis AOx2-type genes was identified. Phylogenetic and expression analyses suggested that AtAOx1d does not belong to the AOx1-type family but forms a new (AOx3-type) family. Tissue-enriched expression profiling revealed that uncoupling protein genes were expressed more ubiquitously than the alternative oxidase genes. Distinct expression patterns among gene family members were observed between monocots and dicots and during chilling stress. These findings suggest that the members of each energy-dissipating system are subject to different cell or tissue/organ transcriptional regulation. As a result, plants may respond more flexibly to adverse biotic and abiotic conditions, in which oxidative stress is involved.
- Sao Paulo State University Brazil
- State University of Campinas Brazil
- Universidade Estadual Paulista - UNESP Brazil
sequence analysis, mitochondrial protein, Arabidopsis, Monocots, Sugar cane, phylogeny, Plants (botany), Ion Channels, sugarcane, mitochondrion, membrane protein, genetics, vegetable protein, mitochondrial uncoupling protein, oxidoreductase, comparative study, Phylogeny, Plant Proteins, messenger RNA, biology, Reverse Transcriptase Polymerase Chain Reaction, Energy dissipation, Mitochondria, Saccharum, Cold Temperature, Multigene Family, sequence alignment, Oxidoreductases, Genome, Plant, Alternative oxidase, enzymology, Molecular Sequence Data, Dicots, reverse transcription polymerase chain reaction, Mitochondrial Proteins, Expression profile, gene expression profiling, Amino Acid Sequence, RNA, Messenger, genome, multigene family, Tissue, Gene Expression Profiling, Proteins, Computational Biology, Membrane Proteins, Plant, cold, amino acid sequence, carrier protein, Genes, Oxidative stress, ion channel, molecular genetics, physiology, Uncoupling proteins, Carrier Proteins, metabolism, Sequence Alignment
sequence analysis, mitochondrial protein, Arabidopsis, Monocots, Sugar cane, phylogeny, Plants (botany), Ion Channels, sugarcane, mitochondrion, membrane protein, genetics, vegetable protein, mitochondrial uncoupling protein, oxidoreductase, comparative study, Phylogeny, Plant Proteins, messenger RNA, biology, Reverse Transcriptase Polymerase Chain Reaction, Energy dissipation, Mitochondria, Saccharum, Cold Temperature, Multigene Family, sequence alignment, Oxidoreductases, Genome, Plant, Alternative oxidase, enzymology, Molecular Sequence Data, Dicots, reverse transcription polymerase chain reaction, Mitochondrial Proteins, Expression profile, gene expression profiling, Amino Acid Sequence, RNA, Messenger, genome, multigene family, Tissue, Gene Expression Profiling, Proteins, Computational Biology, Membrane Proteins, Plant, cold, amino acid sequence, carrier protein, Genes, Oxidative stress, ion channel, molecular genetics, physiology, Uncoupling proteins, Carrier Proteins, metabolism, Sequence Alignment
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