Ca 2+ signalling is central to cochlear sensory hair cell physiology through its influence on sound transduction, membrane filter properties and neurotransmission. However, the mechanism for establishing Ca 2+ homeostasis in these cells remains unresolved. Canonical transient receptor potential (TRPC) Ca 2+ entry channels provide an important pathway for maintaining intracellular Ca 2+ levels. TRPC3 subunit expression was detected in guinea pig and rat organ of Corti by RT‐PCR, and localized to the sensory and neural poles of the inner and outer hair cells (OHCs) by confocal immunofluorescence imaging. A cation entry current with a TRPC‐like phenotype was identified in guinea pig and rat OHCs by whole‐cell voltage clamp. This slowly activating current was induced by the lowering of cytosolic Ca 2+ levels ([Ca 2+ ] i ) following a period in nominally Ca 2+ ‐free solution. Activation was dependent upon the [Ca 2+ ] o and was sustained until [Ca 2+ ] i was restored. Ca 2+ entry was confirmed by confocal fluorescence imaging, and rapidly recruited secondary charybdotoxin‐ and apamin‐sensitive K Ca currents. Dual activation by the G protein‐coupled receptor (GPCR)–phospholipase C–diacylglycerol (DAG) second messenger pathway was confirmed using the analogue 1‐oleoyl‐2‐acetyl‐ sn ‐glycerol (OAG). Ion substitution experiments showed that the putative TRPC Ca 2+ entry current was selective for Na + > K + with a ratio of 1: 0.6. The Ca 2+ entry current was inhibited by the TRPC channel blocker 2‐aminoethyl diphenylborate (2APB) and the tyrosine kinase inhibitor, erbstatin analogue. We conclude that TRPC Ca 2+ entry channels, most likely incorporating TRPC3 subunits, support cochlear hair cell Ca 2+ homeostasis and GPCR signalling.