Healthcare-associated infections (HAIs) caused by the transfer of nosocomial pathogens from high-touch environmental surfaces and medical devices are responsible for significant patient morbidity, mortality and economic cost. An effective cleaning and disinfection practice plays a key role in preventing cross-contaminations and spread of HAIs. Among the most effective surface disinfection methods, the nonwoven ready-to-use disinfectant wipes are increasingly accepted for decontamination of high-touch surfaces because of its convenience and reliable performance. Though some research has been done on the effectiveness of commercial available disinfecting wipes in practical use. Whereas their behaviour during storage remains vague. This project studied the ageing of the disinfecting wipes over storage time. Chloramine as a surface disinfectant and 3 commercial wiping materials of polyester, cellulose, and their combination have been selected. The wipes before and after the contact with disinfectant solution were analysed by FTIR (Fourier-transform infrared spectroscopy) and DMA (Dynamic mechanical analysis).