The m6A reader YTHDF1 regulates axon guidance through translational control of Robo3.1 expression
Copyright policy )The m6A reader YTHDF1 regulates axon guidance through translational control of Robo3.1 expression
N 6-Methyladenosine (m6A) is a dynamic mRNA modification which regulates protein expression in various posttranscriptional levels. Functional studies of m6A in nervous system have focused on its writers and erasers so far, whether and how m6A readers mediate m6A functions through recognizing and binding their target mRNA remains poorly understood. Here, we find that the expression of axon guidance receptor Robo3.1 which plays important roles in midline crossing of spinal commissural axons is regulated precisely at translational level. The m6A reader YTHDF1 binds to and positively regulates translation of m6A-modified Robo3.1 mRNA. Either mutation of m6A sites in Robo3.1 mRNA or YTHDF1 knockdown or knockout leads to dramatic reduction of Robo3.1 protein without affecting Robo3.1 mRNA level. Specific ablation of Ythdf1 in spinal commissural neurons results in pre-crossing axon guidance defects. Our findings identify a mechanism that YTHDF1-mediated translation of m6A-modified Robo3.1 mRNA controls pre-crossing axon guidance in spinal cord.
- Hong Kong Polytechnic University China (People's Republic of)
- Southwest University of Science and Technology China (People's Republic of)
- Southern University of Science and Technology China (People's Republic of)
- Hong Kong University of Science and Technology China (People's Republic of)
- Hong Kong University of Science and Technology (香港科技大學) China (People's Republic of)
Mice, Knockout, Neurons, Adenosine, Gene Expression Regulation, Developmental, Membrane Proteins, RNA-Binding Proteins, Nerve Tissue Proteins, Receptors, Cell Surface, Nervous System, Axons, Axon Guidance, Mice, Spinal Cord, RNA and RNA-protein complexes, Animals, Humans, Protein Binding
Mice, Knockout, Neurons, Adenosine, Gene Expression Regulation, Developmental, Membrane Proteins, RNA-Binding Proteins, Nerve Tissue Proteins, Receptors, Cell Surface, Nervous System, Axons, Axon Guidance, Mice, Spinal Cord, RNA and RNA-protein complexes, Animals, Humans, Protein Binding
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).173 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 0.1%
Citations provided by BIP!Popularity provided by BIP!