Abstract The first detailed biostratigraphic analyses of the Coniacian–middle Campanian shallow-marine carbonate successions exposed in the Mitla Pass, west central Sinai, Egypt have revealed the stratigraphic distribution of diverse calcareous nannofossil and planktonic foraminiferal species. Thirty-six calcareous nannofossils and thirty-two planktonic foraminifera are identified, indicating a Coniacian to middle Campanian age and four Tethyan planktonic foraminiferal and five calcareous nannofossil zones. A comparison of these bioevents from different palaeolatitudes shows considerable variation in age. Three sequence boundaries coincident with the Turonian/Coniacian, Coniacian/Santonian and Santonian/Campanian stage boundaries are recognized. A fourth sequence boundary is marked by a major upper Campanian to early Ypresian (early Eocene) unconformity. These sequence boundaries are primarily related to regional tectonism associated with the Syrian Arc Fold System and secondarily to eustatic sea-level fluctuations.