The fluorescence properties of cerebrospinal fluid (CSF) drawn from the rat cisterna magna, are investigated and compared to the in vivo emission derived from the nucleus raphe dorsalis of the freely moving rat. Measurement in the CSF in ex viva conditions or in situ in the nucleus raphe dorsalis, were realized by way of a fiber optic chemical sensor (FOCS). The in vivo emission, obtained with an excitation wavelength at 337 nm, exhibits 2 peaks around 410 and 460 nm. Absorption spectra, steady-state emission spectra and laser induced time-resolved fluorescence of CSF are reported. With excitation wavelength in the ranges 300-315 nm, 320-355 nm and 360-470 nm, the steady-state emission spectra of CSF exhibit peaks respectively around 340 nm, 390 nm and 530 nm. Despite the limitation attached to the anatomical position, the above data together with those of the literature suggest that in vivo fluorescent signal may be essentially dependent upon intracellular NADH.

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