Abstract. Infiltration is one of the major procedures in water balance research and pollution load estimation in paddy fields. In this study, a new method for indirectly estimating infiltration of paddy fields in situ was proposed and implemented in Taihu Lake basin. Since when there were no rainfall, irrigation and artificial drainage, the water depth variation process of a paddy field is only influenced by evapotranspiration and infiltration (E+F). Firstly, (E+F) was estimated by deciding the steady decreasing rate of water depth; then the evapotranspiration (ET) of the paddy field was calculated by using the crop coefficient method with the recommended FAO-56 Penman-Monteith equation; finally, the infiltration of the paddy field was obtained by subtracting ET from (E+F). Results show that the mean infiltration of the studied paddy field during rice jointing-booting period was 7.41 mm day−1, and the mean vertical infiltration and lateral seepage of the paddy field were 5.46 and 1.95 mm day−1 respectively.