Protocol for unbiased, consolidated variant calling from whole exome sequencing data
Copyright policy )Protocol for unbiased, consolidated variant calling from whole exome sequencing data
Whole Exome Sequencing (WES) is used for querying DNA variants using the protein coding parts of genomes (exomes). However, WES analysis can be challenging because of the complexity of the data. Here, we describe a consolidated protocol for unbiased WES analysis. The protocol uses three variant callers (HaplotypeCaller, FreeBayes, and DeepVariant), which have different underlying models. We provide detailed execution steps, as well as basic variant filtering, annotation, visualization, and consolidation aspects.
Science (General), Bioinformatics, 610, High-Throughput Nucleotide Sequencing, Genomics, Q1-390, Exome Sequencing, Genetics, Protocol, Exome
Science (General), Bioinformatics, 610, High-Throughput Nucleotide Sequencing, Genomics, Q1-390, Exome Sequencing, Genetics, Protocol, Exome
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).4 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!