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Bisulfite-free and base-resolution analysis of 5-methylcytidine and 5-hydroxymethylcytidine in RNA with peroxotungstate

Authors: Fang Yuan; Ying Bi; Paulina Siejka-Zielinska; Ying-Lin Zhou; Xin-Xiang Zhang; Chun-Xiao Song;
APC: 1,395.87 EUR

Bisulfite-free and base-resolution analysis of 5-methylcytidine and 5-hydroxymethylcytidine in RNA with peroxotungstate

Abstract

WO-Seq: a bisulfite-free and base-resolution sequencing method based on peroxotungstate oxidation is presented for the identification of hm5C sites in the transcriptome. Combining the peroxotungstate oxidation with TET enzyme oxidation, m5C can also be detected in a procedure termed TET-Assisted WO-Seq (TAWO-Seq).

Country
United Kingdom
Related Organizations
Keywords

Chemistry, Base Sequence, Sequence Analysis, RNA, RNA, Cytidine, Tungsten Compounds

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    influence
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
39
Top 10%
Top 10%
Top 10%
Green
hybrid