Cucumber mosaic virus-associated RNA 5
Copyright policy )Cucumber mosaic virus-associated RNA 5
Two satellite RNA of cucumber mosaic virus (CMV) designated J876-CARNA-5 (for cucumber mosaic virus-associated RNA-5) and D27-CARNA-5 have been molecularly and biologically characterized. J876-CARNA-5 (387 nucleotides (nt)) and D27-CARNA-5 (391 nt) have nearly identical 5' half insertion/deletion regions where 120 nt replace approximately 70 nt of D-CARNA-5 (335 nt), the first variant described and sequenced. J876-CARNA-5 possesses the 15-nt conserved sequence element in its 3' half which is present in all tomato necrogenic variants and induces the same level of necrosis in tomatoes as the prototype necrogenic D-CARNA-5. D27-CARNA-5 lacks the 3' half necrosis-determining element and attenuates the CMV symptoms in tomato. Transcripts of cloned cDNA of J876-CARNA-5 were stably propagated in tomato in the presence of CMV-1. Purified J876-CARNA-5 progeny, inoculated with CMV-1 in a quantitative bioassay, induced tomato necrosis at the same dilution level as the natural satellite. Several computer-generated secondary structures of CMV satellites were examined and the possible correlation of a defined secondary structural element with necrosis induction is discussed.
- United States Department of Agriculture United States
- Agricultural Research Service United States
Electrophoresis, Base Sequence, Transcription, Genetic, Molecular Sequence Data, Cucumovirus, Satellite Viruses, Nucleic Acid Conformation, RNA, Viral, Plant Diseases
Electrophoresis, Base Sequence, Transcription, Genetic, Molecular Sequence Data, Cucumovirus, Satellite Viruses, Nucleic Acid Conformation, RNA, Viral, Plant Diseases
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