Introduction: Age estimation in children and adolescents often depends on morphological methods, such as examination of dental development. Objectives: The aim of this project was to validate, in a Portuguese population, two forensic methods of dental age estimation – Schour and Massler charts and the London atlas. Materials and Methods: The test sample was composed by 108 dental radiographs of living and known-age individuals. Dental age was estimated according to each method. Chronological age was then compared to the estimated dental age using individual t-test and paired t-test. Results: Results showed that the Schour and Massler charts underestimated age and the London atlas overestimated age. Nevertheless, the London atlas performed better in all measures. Mean differences for both the London atlas and Schour and Massler were 0,1389 and -5,4167 months respectively. Schour and Massler charts showed significant statistical difference between dental age and chronological age (p <0.05). Discussion and Conclusions: We conclude that, in the evaluated sample, age estimation using the London atlas represents an improvement in forensic age estimation from developing teeth. Further studies should be done with a larger Portuguese population sample.