Abstract The variability of hyperiid amphipod assemblages was analyzed based on seasonal (dry vs. wet) and spatial differences (neritic vs. oceanic) in the Panama Bight. Four oceanographic cruises were carried out during 2007–2008, two during the dry season (Feb. 2007 and Mar. 2008) and two during the wet season (Sep. 2007 and Sep. 2008). Cooler ( 34 psu) at 30 m were observed during the dry season, while the opposite pattern occurred during the wet season (>25 °C, Hyperioides sibaginis and Lestrigonus bengalensis being the most abundant (76%). The structure of the hyperiids assemblages was similar between seasons; this may be due to the interannual variability associated with two ENSO processes (El Nino: Feb. 2007; La Nina: Sep. 2007 and Mar. 2008). Spatially, the structure of the hyperiids assemblages changed during the wet season, as a consequence of the strong neritic–oceanic contrast in salinity, while it was spatially similar during the dry season, as a consequence of species penetration toward the coast promoted by colder and saltier waters. The abundance of hyperiids was significantly correlated with the abundance of gelatinous zooplankton (siphonophores, ctenophores, doliolids and salps), which are commonly used as hosts by this group.