90S Pre-Ribosomes Include the 35S Pre-rRNA, the U3 snoRNP, and 40S Subunit Processing Factors but Predominantly Lack 60S Synthesis Factors
Copyright policy )90S Pre-Ribosomes Include the 35S Pre-rRNA, the U3 snoRNP, and 40S Subunit Processing Factors but Predominantly Lack 60S Synthesis Factors
We report the characterization of early pre-ribosomal particles. Twelve TAP-tagged components each showed nucleolar localization, sedimented at approximately 90S on sucrose gradients, and coprecipitated both the 35S pre-rRNA and the U3 snoRNA. Thirty-five non-ribosomal proteins were coprecipitated, including proteins associated with U3 (Nop56p, Nop58p, Sof1p, Rrp9, Dhr1p, Imp3p, Imp4p, and Mpp10p) and other factors required for 18S rRNA synthesis (Nop14p, Bms1p, and Krr1p). Mutations in components of the 90S pre-ribosomes impaired 40S subunit assembly and export. Strikingly, few components of recently characterized pre-60S ribosomes were identified in the 90S pre-ribosomes. We conclude that the 40S synthesis machinery predominately associates with the 35S pre-rRNA factors, whereas factors required for 60S subunit synthesis largely bind later, showing an unexpected dichotomy in binding.
- University of Edinburgh United Kingdom
- University of Geneva Switzerland
- Wellcome Centre for Cell Biology United Kingdom
Macromolecular Substances, Blotting, Western, 612, Cell Biology, Blotting, Northern, Fungal Proteins, Molecular Weight, Protein Subunits, RNA, Ribosomal, Ribonucleoproteins, Small Nucleolar, Yeasts, Centrifugation, Density Gradient, RNA Precursors, Electrophoresis, Polyacrylamide Gel, Molecular Biology, Ribosomes, ddc: ddc:612
Macromolecular Substances, Blotting, Western, 612, Cell Biology, Blotting, Northern, Fungal Proteins, Molecular Weight, Protein Subunits, RNA, Ribosomal, Ribonucleoproteins, Small Nucleolar, Yeasts, Centrifugation, Density Gradient, RNA Precursors, Electrophoresis, Polyacrylamide Gel, Molecular Biology, Ribosomes, ddc: ddc:612
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