Micellar gradients in size-exclusion simulated moving bed chromatography

descriptionPublicationkeyboard_double_arrow_right Article 01 Apr 2006 Netherlands English Publisher:Elsevier BVJournal:Journal of Chromatography A, volume 1,113, pages 130-139 (issn: 0021-9673,

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Authors: Horneman, D.A.; Ottens, M.; Keurentjes, J.T.F.; Wielen, van der, L.A.M.;

doi: 10.1016/j.chroma.2006.01.134

pmid: 16504200

Micellar gradients in size-exclusion simulated moving bed chromatography

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Abstract

The selectivity of size-exclusion chromatography (SEC) can be modified by adding non-ionic micelles to the mobile phase. Surfactant-aided size-exclusion chromatography (SASEC) can therefore very well be performed in a gradient mode on an SMB, as is reported in this paper. A method has been developed for correctly positioning a micellar gradient over an SMB. The method is applied for size-exclusion chromatography with the non-ionic surfactant C12E23 as gradient forming solute, and demonstrated by applying it to a relevant chromatographic protein separation problem.

Country

Netherlands

Related Organizations

Delft University of Technology
Netherlands
Eindhoven University of Technology
Netherlands
Technical University Eindhoven
Netherlands

Keywords

Immunoglobulin G, Chromatography, Gel, Dimerization, Micelles

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