ABSTRACTBackground:The short-lived fishNothobranchius furzeriis the shortest-lived vertebrate that can be cultured in captivity and was recently established as a model organism for aging research. Small non-coding RNAs, especially miRNAs, are implicated in age-dependent control of gene expression.Results:Here, we present a comprehensive catalogue of miRNAs and several other non-coding RNA classes (ncRNAs) forNothobranchius furzeri. Analyzing multiple small RNA-Seq libraries, we show most of these identified miRNAs are expressed in at least one of sevenNothobranchiusspecies. Additionally, duplication and clustering ofN. furzerimiRNAs was analyzed and compared to the four fish speciesDanio rerio,Oryzias latipes,Gasterosteus aculeatusandTakifugu rubripes. A peculiar characteristic ofN. furzerias compared to other teleosts was a duplication of the miR-29 cluster.Conclusion:The completeness of the catalogue we provide is comparable to that of zebrafish. This catalogue represents a basis to investigate the role of miRNAs in aging and development in this species.Availability:All supplementary material can be found online athttp://www.rna.uni-jena.de/en/supplements/nothobranchius-furzeri-mirnome/.