The calcium-activated nonselective cation channel TRPM4 is essential for the migration but not the maturation of dendritic cells
Copyright policy )The calcium-activated nonselective cation channel TRPM4 is essential for the migration but not the maturation of dendritic cells
Dendritic cell (DC) maturation and migration are events critical for the initiation of immune responses. After encountering pathogens, DCs upregulate the expression of costimulatory molecules and subsequently migrate to secondary lymphoid organs. Calcium (Ca(2+)) entry governs the functions of many hematopoietic cell types, but the role of Ca(2+) entry in DC biology remains unclear. Here we report that the Ca(2+)-activated nonselective cation channel TRPM4 was expressed in and controlled the Ca(2+) homeostasis of mouse DCs. The absence of TRPM4, which elicited Ca(2+) overload, did not influence DC maturation but did considerably impair chemokine-dependent DC migration. Our results establish TRPM4-regulated Ca(2+) homeostasis as crucial for DC mobility but not maturation and emphasize that DC maturation and migration are independently regulated.
- Inserm France
- University of Lille France
- University of Paris France
- Paris 13 University France
- Université de Caen Normandie France
Mice, Knockout, Patch-Clamp Techniques, Immunoblotting, Gene Expression, TRPM Cation Channels, Bone Marrow Cells, Cell Differentiation, Dendritic Cells, Flow Cytometry, Mice, Cell Movement, Animals, Homeostasis, Calcium Signaling
Mice, Knockout, Patch-Clamp Techniques, Immunoblotting, Gene Expression, TRPM Cation Channels, Bone Marrow Cells, Cell Differentiation, Dendritic Cells, Flow Cytometry, Mice, Cell Movement, Animals, Homeostasis, Calcium Signaling
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).187 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!