KDML 105 is the famous fragrant rice of Thailand. 2AP acts as the characteristic compounds in fragrant-rice cultivars. 2AP accumulation has been reported in relationship with proline content and the growth rate of rice. Several factors have been reported to affect the poline and 2AP contents. We determined the combinatorial effect of gamma irradiation and salt concentration on the 2AP and proline contents of 5 day-old KDML 105 rice-seedling. In the growth condition under 20 mM of NaCl concentration the proline content of gamma irradiated rice increased, compared to the gamma-irradiated rice treated without 20 mM of NaCl concentration. While, the shoot-length of 5 day-old gamma irradiated rice-seedlings under 20mM NaCl concentration cannot be observed when compared to gamma irradiated rice-seedling without salt concentration. The 2AP content of gamma irradiated rice-seedlings under 20 mM of NaCl concentration had approximately 2.6 to 3.1 times higher than in the growth condition without salt concentration of gamma irradiated rice-seedlings. Our results indicate that the combination of gamma irradiation technique and salt concentration can be used for improving the 2AP content in rice.