The study of the chemical composition of biologically active substances (neutral lipids and their fatty acid composition, prothenogenic amino acids, alkaloids) and the content of macro- and microelements in the shoots of three types of liverworts from the flora of the Komi Republic – Tetralophosia setiformis (Ehrh.) Schljakov, Ptilidium pulcherrimum (Weber) Vain. and Radula complanata (L.) Dumort, was carried out. It is shown that the content of neutral lipids in the studied representatives lies in the lower limits typical for plants (0.3; 0.9; 0.5% of dry weight). The fatty acid composition of neutral lipids is represented by molecules with chains from C12 to C20. It was found that the main share is made up of saturated fatty acids, among which palmitic (C16:0), stearic (C18:0) and arachinic (C20:0) dominate. Unsaturated acids are mainly represented by oleic and linoleic acids. Lipids of P. pulcherrimum are characterized by a fairly high content of extremely rare unsaturated vaccenic acid in plants (3.0% of the total acid content), which is classified as an unusual fatty acid. The mass fraction of nitrogen in the dry matter of three types of liverworts ranges from 0.83 to 1.27%. Of the 17 proteinogenic acids in all liver samples, the dominant ones are aspartic and glutamic acids, which are part of the proteins of all known living organisms, as well as alanine, leucine and lysine. In addition, a fairly high content of the partially interchangeable amino acid arginine was found. In the three studied species, 13 micro- and six macronutrients were found. At the same time, T. setiformis is characterized by a higher content of trace elements of iron, aluminum and lead and the macronutrient sodium, P. pulcherrimum – manganese, potassium and phosphorus, R. complanata – trace elements of zinc, copper, barium and strontium, macronutrients of calcium and magnesium. Testing the samples for the presence of alkaloids using sedimentary reactions and UV detection of extracts gave a negative result for two species, but a positive reaction with two reagents and an intense glow during UV detection of P. pulcherrimum extracts, which requires additional more detailed study.