AbstractFluchloralin [N‐(2‐chloroethyl)‐α,α,α,‐trifluoro‐2,6‐dinitro‐N‐propyl‐p‐toluidine] was transformed by two common soil fungi, Aspergillus flavus Link and Fusarium solani (Mart.) Sacc. in pure culture. The fungi were isolated from enrichment cultures of an analogous herbicidal compound, pendimethalin [N‐(1‐ethylpropyl)‐2,6‐dinitro‐3,4‐xylidine)]. Transformation of fluchloralin by these fungi resulted in the formation of several metabolites, 13 of which were identified. Besides the known mechanisms of microbial transformation of dinitroanilines, methylation of the anilino nitrogen and direct elimination of a nitro group at 2 position from the aromatic ring without any further substitution were also observed.