A peer-reviewed antibody characterization article corresponding to this Zenodo preprint is openly available at F1000Research: https://doi.org/10.12688/f1000research.140456.1 This report presents a guide to selecting high-quality commercial antibodies against Tyrosine-protein kinase SYK by immunoblot (Western blot), immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. This work was supported in part by the Emory-Sage-SGC TREAT-AD center established by the National Institutes of Aging under award number U54AG065187.