Expression of STAT5, COX-2 and PIAS3 in Correlation with NSCLC Histhopathological Features
Copyright policy )Expression of STAT5, COX-2 and PIAS3 in Correlation with NSCLC Histhopathological Features
Signal transducers and activators of transcription (STATs), their inhibitors and cyclooxygenase-2 (COX-2) participate in transformations of many various types of cancers. The aim of the present study was to evaluate the relationship between STAT5A/B, COX-2, and PIAS3 mRNA expression and tumor staging, metastasis status, and histopathological subtype in 71 patients with confirmed non-small cell lung cancer (NSCLC) diagnosis. Total RNA was isolated from NSCLC tissue samples and the expression of the studied genes was assessed using TaqMan probes in real-time PCR assay. The expression levels of STAT5A, STAT5B, and COX-2 genes were increased in 69%, 79%, and 71% NSCLC samples respectively, while PIAS3 expression was decreased in the majority (69%) of the studied tissues. Statistically significant differences were observed between STAT5 isoforms (P = 0.0008), with higher expression of STAT5B. We found statistically significant positive correlation between STAT5B and COX-2 (rho = 0.045), and significant negative correlation between STAT5B and PIAS3 (rho = -0.049). The negative correlation between STAT5B and PIAS3 (rho = -0.43) was also observed in T2a+T2b tumor group. Additionally, STAT5B and COX-2 expression levels were significantly different between T1a+T1b and T2a+T2b tumors (P = 0.002 and P = 0.041, respectively), with higher expression of both genes in T2 tumor stage. PIAS3 expression was significantly lower in NSCC subtype as compared with SCC subtype (P = 0.017). Also, STAT5A and STAT5B immunoexpression was assessed, and the results indicated significantly higher protein levels in NSCLC patients as compared with controls (P = 0.048 and P = 0.034, respectively). High STAT5B immunoexpression was positively correlated with STAT5B gene expression in tumors (rho = 0.755). STAT5B protein level was also significantly higher in T2a+T2b tumors, reflecting high STAT5B gene expression in this group. There was no statistically significant association between mRNA and protein expression levels of the studied genes and patients' characteristics: age, gender, smoking. The obtained results highlight the importance of the genes STAT5B and COX-2 in lung cancer progression.
Male, Lung Neoplasms, Science, Risk Factors, Carcinoma, Non-Small-Cell Lung, STAT5 Transcription Factor, Humans, Protein Isoforms, RNA, Messenger, Aged, Neoplasm Staging, Q, Smoking, R, Middle Aged, Protein Inhibitors of Activated STAT, Gene Expression Regulation, Neoplastic, Cell Transformation, Neoplastic, Cyclooxygenase 2, Lymphatic Metastasis, Disease Progression, Medicine, Female, Research Article, Molecular Chaperones
Male, Lung Neoplasms, Science, Risk Factors, Carcinoma, Non-Small-Cell Lung, STAT5 Transcription Factor, Humans, Protein Isoforms, RNA, Messenger, Aged, Neoplasm Staging, Q, Smoking, R, Middle Aged, Protein Inhibitors of Activated STAT, Gene Expression Regulation, Neoplastic, Cell Transformation, Neoplastic, Cyclooxygenase 2, Lymphatic Metastasis, Disease Progression, Medicine, Female, Research Article, Molecular Chaperones
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