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image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Wound Repair and Reg...arrow_drop_down
image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
Wound Repair and Regeneration
Article . 2017 . Peer-reviewed
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Foxn1 and Mmp‐9 expression in intact skin and during excisional wound repair in young, adult, and old C57Bl/6 mice

Authors: Barbara Gawronska-Kozak; Joanna Bukowska; Anna Kur-Piotrowska; Jeffrey M. Gimble; Marta Kopcewicz;

Foxn1 and Mmp‐9 expression in intact skin and during excisional wound repair in young, adult, and old C57Bl/6 mice

Abstract

AbstractThe transcription factor Foxn1 is essential for skin development. Our previous studies performed on young C57BL/6J mice model showed that Foxn1 acts as regulator of the skin wound healing process. The present study extended our initial research regarding the expression and potential role of Foxn1 in the intact and wounded skin as a function of animal age and stage of the wound healing process. We analyzed Foxn1 and Mmp‐9 expression in the intact and postinjured skin of young, adult, and old C57BL/6J and transgenic Foxn1::Egfp mice. The similar levels of epidermal Foxn1 mRNA expression were detected in young and adult C57BL/6J mice and higher levels in old animals. Postinjured skin tissues displayed a gradual decrease of Foxn1 mRNA expression at Days 1, 5, and 7 after injury. Foxn1‐eGFP positive cells were abundant at wound margin and in re‐epithelialized epidermis at postwounded Days 1, 5, and 7 and colocalized with E‐cadherin and Mmp‐9. Postwounded skin at Days 14–36 displayed Foxn1‐eGFP cells in the epidermis and in the dermal part of the skin (papillary dermis). A subset of Foxn1‐eGFP positive cells in the papillary dermis expressed the myofibroblast marker αSMA. Flow cytometric analysis of cells isolated from postwounded (Day 7) skin tissues showed a significant increase in the percentage of Foxn1‐eGFP positive cells with phenotype of double positivity for E‐cadherin/N‐cadherin (epithelial/mesenchymal markers). Collectively, these data identify the transcription factor Foxn1 as a potential key epidermal regulator modifying both epidermal and dermal healing processes after cutaneous wounding.

Keywords

Keratinocytes, Wound Healing, Epithelial-Mesenchymal Transition, Age Factors, Forkhead Transcription Factors, Mice, Transgenic, Immunohistochemistry, Mice, Inbred C57BL, Disease Models, Animal, Mice, Gene Expression Regulation, Matrix Metalloproteinase 9, Animals, Signal Transduction, Skin

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
19
Top 10%
Average
Top 10%
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