The ceramide-activated protein phosphatase Sit4p controls lifespan, mitochondrial function and cell cycle progression by regulating hexokinase 2 phosphorylation
Copyright policy )The ceramide-activated protein phosphatase Sit4p controls lifespan, mitochondrial function and cell cycle progression by regulating hexokinase 2 phosphorylation
Sit4p is the catalytic subunit of a ceramide-activated PP2A-like phosphatase that regulates cell cycle, mitochondrial function, oxidative stress resistance and chronological lifespan in yeast. In this study, we show that hexokinase 2 (Hxk2p) is hyperphosphorylated in sit4Δ mutants grown in glucose medium by a Snf1p-independent mechanism and Hxk2p-S15A mutation suppresses phenotypes associated with SIT4 deletion, namely growth arrest at G1 phase, derepression of mitochondrial respiration, H2O2 resistance and lifespan extension. Consistently, the activation of Sit4p in isc1Δ mutants, which has been associated with premature aging, leads to Hxk2p hypophosphorylation, and the expression of Hxk2p-S15E increases the lifespan of isc1Δ cells. The overall results suggest that Hxk2p functions downstream of Sit4p in the control of cell cycle, mitochondrial function, oxidative stress resistance and chronological lifespan.
Saccharomyces cerevisiae Proteins, Hydrogen Peroxide, Saccharomyces cerevisiae, Phosphoproteins, G1 Phase Cell Cycle Checkpoints, Mitochondria, Glucose, Oxygen Consumption, Gene Expression Regulation, Fungal, Hexokinase, Mutation, Protein Phosphatase 2, Phosphorylation, Gene Deletion, Metabolic Networks and Pathways, Signal Transduction
Saccharomyces cerevisiae Proteins, Hydrogen Peroxide, Saccharomyces cerevisiae, Phosphoproteins, G1 Phase Cell Cycle Checkpoints, Mitochondria, Glucose, Oxygen Consumption, Gene Expression Regulation, Fungal, Hexokinase, Mutation, Protein Phosphatase 2, Phosphorylation, Gene Deletion, Metabolic Networks and Pathways, Signal Transduction
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).21 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!