The micronutrients and biostimulant use can promote root, shoot and seedling growth. The aim of this work was evaluated the micronutrients and biostimulant application effects on arabica coffee seedling development. The experimental design was a randomized block in factorial 5 x 3, with four replicates. Five cultivars of arabica coffee (Topazio, Catuai Amarelo, Catuai Vermelho 99, Catuai Vermelho 144 and Catigua) cultivated in green house, combined with biostimulant (Stimulate®), of micronutrients (Mo+Co) and control. 150 days after sowing the plants was evaluated. The higher seed germination velocity (0.10) was obtained with micronutrients and biostimulant. The higher height of plant, 8.88 cm, was attained with biostimulant on Catuai Amarelo. Catuai Amarelo and Vermelho obtained higher number of leaves (4.5) with biostimulant use and the Catuai Amarelo (4.38) with micronutrients use. In Catuai Amarelo was attained higher shoot dry mass and leaf area with biostimulant use, reach 1.69 g and 19.55 cm 2 , respectively. The higher root dry mass values (0.76 g) and Dickson quality index (0.47 and 0.48) was attained with micronutrients use to the Catuai Amarelo e vermelho cultivars. Seedling development of Catuai Amarelo was benefited with Stimulate® application and the cultivar Topazio did not get positive development with Stimulate® and micronutrients application.