
Additional file 3: Supplement-Figure 3. Old mice have reduced peritoneal myeloid cells. Peritoneal lavage from young (average age of 9 weeks, n=5) or old (average age of 113 weeks) mice was stained with antibodies against F4/80, CD11b, CD19, CD90 and CD45 and analyze by flow cytometry. a) Gating strategy. b) Distribution of subsets among living CD45+ is shown in the pie charts: myeloid cells = CD45+ and F4/80+ or CD11b+, T cells: CD45+F4/80-CD11b-CD90+; B cells: CD45+F4/80-CD11b-CD90-CD19+. Statistical testing was performed as described in the methods section.
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