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Discrete Flow Cytometry of Underway Samples from Gradients 4 (2021) Using a BD Influx Cell Sorter

Research datakeyboard_double_arrow_right Dataset 17 May 2022 English Publisher:Zenodo

Authors: Cain, Kelsy; Ribalet, François; Armbrust, Virginia;

doi: 10.5281/zenodo.6014650 , 10.5281/zenodo.6558008

Discrete Flow Cytometry of Underway Samples from Gradients 4 (2021) Using a BD Influx Cell Sorter

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Abstract

The dataset consists of BD Influx-based analysis of phytoplankton populations from discrete flow cytometry data collected underway during the Gradients 2021 (Gradients 4/TN397) oceanographic research cruise in the equatorial Pacific Ocean. The analysis includes cell abundance, forward light scatter, and pigment fluorescence of individual cells, including picoeukaryotes and the cyanobacteria Prochlorococcus and Synechococcus. The analysis also includes estimates of cell size, carbon content, and biomass using forward light scatter values and Mie Theory. Time is in UTC format, latitude and longitude are in decimal degrees, and depth is in meters. Further information can be found here: https://github.com/fribalet/FCSplankton

Keywords

synechococcus, flow cytometry, phytoplankton, prochlorococcus, picoeukaryotes, BD Influx, Simons CMAP

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