AbstractThe effect of atmospheric change on birch aphid (Euceraphis betulae Koch) oviposition preference was examined and plant characteristics that are possibly responsible for the observed effects were investigated. It was hypothesized that the increasing concentrations of CO2 and O3 affect singly or in combination the oviposition of birch aphids via changes in host plant characteristics. Two genotypes of field‐growing silver birch (Betula pendula Roth) trees (clones 4 and 80), which were exposed to doubled ambient concentration of CO2 and O3, singly and in combination, in a 3‐year open‐top chamber experiment, were used in an aphid oviposition preference test. It was found that elevated CO2, irrespective of ozone concentration, increased the number of aphid eggs laid on clone 4, but not in clone 80. Several flavonoid aglycones were identified from the exudate coating of birch buds. Although elevated CO2 and O3 affected these phenolic compounds in clone 4, the effects did not correlate with the observed changes in aphid oviposition. It is suggested that neither bud length, which was not affected by the treatments, nor surface exudate phenolics mediate birch aphid oviposition preference.