Double-Stranded RNA Adenosine Deaminase ADAR-1-Induced Hypermutated Genomes among Inactivated Seasonal Influenza and Live Attenuated Measles Virus Vaccines
Copyright policy )Double-Stranded RNA Adenosine Deaminase ADAR-1-Induced Hypermutated Genomes among Inactivated Seasonal Influenza and Live Attenuated Measles Virus Vaccines
ABSTRACT We sought to examine ADAR-1 editing of measles and influenza virus genomes derived from inactivated seasonal influenza and live attenuated measles virus vaccines grown on chicken cells as the culture substrate. Using highly sensitive 3DI-PCR (R. Suspène et al., Nucleic Acids Res. 36:e72, 2008), it was possible to show that ADAR-1 could hyperdeaminate adenosine residues in both measles virus and influenza virus A genomes. Detailed analysis of the dinucleotide editing context showed preferences for 5′ArA and 5′UrA, which is typical of editing in mammalian cells. The hyperedited mutant frequency, including genomes and antigenomes, was a log greater for influenza virus compared to measles virus, suggesting a greater sensitivity to restriction by ADAR-1.
Base Sequence, Adenosine Deaminase, Measles Vaccine, Molecular Sequence Data, RNA-Binding Proteins, Genome, Viral, Vaccines, Attenuated, Cell Line, Influenza A virus, Influenza Vaccines, Measles virus, Chlorocebus aethiops, Influenza, Human, Mutation, Animals, Humans, Seasons, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, Vero Cells, Measles
Base Sequence, Adenosine Deaminase, Measles Vaccine, Molecular Sequence Data, RNA-Binding Proteins, Genome, Viral, Vaccines, Attenuated, Cell Line, Influenza A virus, Influenza Vaccines, Measles virus, Chlorocebus aethiops, Influenza, Human, Mutation, Animals, Humans, Seasons, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, Vero Cells, Measles
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