This is a list of herbaceous and arboreous plants found at the Komaba Campus of University of Tokyo, Japan, and recorded between 2017 to 2019. All occurrences were recorded as voucher specimens, which were deposited at the Komaba Museum, University of Tokyo, Meguro City, Japan (KMUT). In total, 324 specimens were registered, identified under 99 families and 236 genera. The Komaba Campus of University of Tokyo has an area of about 25.4ha and is located at a highly urbanized area in Tokyo Metropolis (Japan), under a temporal zone (35.6N, 139.7E). Although almost all areas of the campus are covered by artificial constructions (e.g., research buildings, road, and athletic field), small green spaces, with an approximate area of 4.5ha, are present. Although many non-native species are observed in this area and peripheral areas due to urbanization, some new insect species had been recorded in this campus in recent year. Therefore, some surveys of fauna were performed on this campus in the last decade, but the only flora survey available for the area was performed 35 years ago. It is suggested that the area and position of green space in the Komaba campus has changed over these 35 years, and hence an updated survey is made necessary.