The dataset comprises 1970 occurrences of 379 vascular plant species collected during the DarkDivNet sampling in Kamianska Sich National Nature Park (Berislav District, Kherson Region, Ukraine). The fieldwork for this project was conducted in May-June 2021 as a contribution to the global network which aims to explore the dark diversity of plant communities (DarkDivNet). Following the DarkDivNet methodology (Lewis et al. 2016), we established two permanent core plots and 32 co-occurrence plots within a monitoring area that had a radius of 10 km. Then we recorded all vascular plant species within each plot (shoot presence). The surveyed locations cover all types of grasslands and steppic shrubs represented within the study area. The nomenclature of the dataset follows “Vascular Plants of Ukraine: A Nomenclatural Checklist” (Mosyakin & Fedoronchuk 1999).