A dry medium formulation which provides the growth and reproduction of Leishmania promastigotes was designed. The medium has been prepared by using nondietary protein raw material, it contains domestic-production ingredients of that in high supply. The nutrient basis of the medium is enzymatic peptone (a source of amine nitrogen). The growth factors of Leishmania (a yeast extract, vitamins, amino acids, a dry blood derivative), carbohydrates (an energy components of the medium), inorganic salts (a source of trace elements), which are selected in strictly defined quantitative ratios, meet the physiological requirements of Leishmania. The agent rules out the necessity of adding ex tempore blood, making the process for preparing the medium from the dry powder simple and easy-to-use.