[Comparative studies of the detection of antibodies against BHV-5 using the enzyme-linked immunosorbent assay, serum neutralization test, cell assay and immunofluorescence test].

A, Lindner; H, Ambrosius; H, Liebermann

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Article . 1994

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[Comparative studies of the detection of antibodies against BHV-5 using the enzyme-linked immunosorbent assay, serum neutralization test, cell assay and immunofluorescence test].

descriptionPublicationkeyboard_double_arrow_right Article 25 Jan 1994 German Journal:DTW. Deutsche tierarztliche Wochenschrift, volume 100 (issn: 0341-6593,

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Authors: A, Lindner; H, Ambrosius; H, Liebermann;

pmid: 8261916

[Comparative studies of the detection of antibodies against BHV-5 using the enzyme-linked immunosorbent assay, serum neutralization test, cell assay and immunofluorescence test].

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Abstract

A comparison of the analyses of 490 serum samples from various flocks of sheep for the detection of BHV-5 antibodies using ELISA, serum neutralisation test, cell assay and immunofluorescence assay showed nearly identical results regarding the distinction between positive and negative serum samples. The percentage of serum samples with antibodies against BHV-5 antigen differed from test to test: ELISA 268 (54.7%), cell assay 323 (65.8%), immunofluorescence assay 222 (46.6%) and serum neutralisation test 221 (45.1%). With ELISA, cell assay and immunofluorescence assay the experiment can be carried out more simply and rapidly than with serum neutralisation test.

Related Organizations

Leipzig University
Germany

Keywords

Sheep, Neutralization Tests, Animals, Fluorescent Antibody Technique, Sheep Diseases, Enzyme-Linked Immunosorbent Assay, Herpesviridae Infections, Antibodies, Viral, Herpesviridae

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