Measurement of phosphotyrosine phosphatase activity using the Folin-Ciocalteu phenol reaction.
Copyright policy )Measurement of phosphotyrosine phosphatase activity using the Folin-Ciocalteu phenol reaction.
An assay for the estimation of phosphotyrosine phosphatase using the Folin-Ciocalteu phenol reaction to monitor enzyme activity is presented. The method is based on the property of the substrate phosphotyrosine not to react as a phenol until it is dephosphorylated. The method is sensitive, there is no interference from the use of detergents and it does not rely on special laboratory equipment to distinguish tyrosine from phosphotyrosine.
- University of Vienna Austria
Molybdenum, Carcinoma, Molecular Sequence Data, Tungsten Compounds, Sensitivity and Specificity, Rats, Liver, Colonic Neoplasms, Tumor Cells, Cultured, Animals, Tyrosine, Indicators and Reagents, Amino Acid Sequence, Protein Tyrosine Phosphatases
Molybdenum, Carcinoma, Molecular Sequence Data, Tungsten Compounds, Sensitivity and Specificity, Rats, Liver, Colonic Neoplasms, Tumor Cells, Cultured, Animals, Tyrosine, Indicators and Reagents, Amino Acid Sequence, Protein Tyrosine Phosphatases
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