[Spectrophotometric evaluation of diene conjugates].

Primary products of lipid peroxidation in liver tissue (diene conjugates) were extracted using various systems of solvents. In the system heptane-isopropanol 1:1 the diene conjugates were mainly extracted by the alcohol. The procedure for estimation of the tissue diene conjugates involved extraction with heptane-isopropanol, purification and spectrophotometric measurement of the extinction in the alcohol fraction.

Keywords

Lipid Peroxides, Spectrophotometry, Microsomes, Liver, Animals, 1-Propanol, Heptanes, Rats

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