Chronic kidney disease (CKD) is a global health problem with increasing prevalence. This study aims to analyze the effect of Ramadan fasting on important biomarkers in CKD patients.A prospective cohort study was conducted on 30 CKD patients with stages 1, 2, and 3A who underwent Ramadan fasting. Measurements of MDA, PARP, SIRT1, NR1D1, and TGF-β levels were carried out before fasting, 2 weeks during fasting, and after fasting using the Enzyme-linked immunosorbent assay (ELISA) method.There were significant decreases in urea, fasting blood glucose, HbA1C, and uric acid levels (p<0.05). MDA and SIRT1 decreased significantly (p<0.001), while PARP and NR1D1 increased significantly (p<0.001). TGF-β also showed a decrease. There were no significant changes in lipid profiles, creatinine, and albumin.Ramadan fasting has significant effects on several biochemical parameters and biological markers in early-stage CKD patients. These changes indicate potential improvements in oxidative stress, cell autophagy, inflammation regulation, and circadian rhythm. Further studies are needed to evaluate the long-term effects and clinical implications of these findings in CKD management.