Tillage and straw incorporation are important agricultural practices that can break plough layer and improve black soil fertility. The effects of tillage and straw incorporation on straw humification coefficient, soil organic carbon (SOC), and maize yield were investigated in a field experiment. Subsoil combined with straw incorporation in 20-35 cm soil layer (ST+S) could break plough layer and decrease the bulk density by 5.7%, 3.3% and 5.7% compared with traditional til-lage (TT), subsoil (ST) and traditional tillage combined with straw incorporation (TT+S) in six experimental years, respectively, and the best effects were observed in ST and ST+S treatments in the first expe-rimental year. The rate of straw decomposition was higher in 0-20 cm (72.0%) than in 20-35 cm (59.2%), and the straw humification coefficient in 0-20 cm and 20-35 cm soil la-yers reached the peak in first experimental year with 15.9% and 12.7%, respectively. Compared with initial soil sample, SOC and light fraction organic carbon (LFOC) of TT, ST and ST+S treatments in 0-20 cm soil layer was decreased in experimental years, but was increased by 2.9% and 12.4% within TT+S, respectively. SOC and light fraction organic carbon (LFOC) of ST+S in 20-35 cm soil layer was increased by 9.2% and 9.9%, respectively. The effect of field treatments on maize yield showed in a decreasing trend of ST+S>TT+S>ST>TT, effects of tillage and straw incorporation on maize yield could continue 3 and 6 years, respectively, indicating that tillage and straw incorporation had time effect. Therefore, straw incorporated into 20-35 cm soil layer based on tillage was an effective, sustainable agricultural practice of improving black soil quality.