Flavanones are an important group of flavonoids that are characteristic for citrus. In the present work isolation of flavanones from mandarin peel was performed by conventional extraction using water, ethanol, acetone and aqueous solutions of acetone and ethanol. The extracts were analysed on the content and composition of flavanones. Furthermore the DPPH radical scavenging activity of extracts was determined. Finally, the influence of extraction parameters (particle size, extraction temperature, extraction time,material to solvent ratio, number of extraction stages and type of solvent) on the yield and the efficiency of extraction were determined by Plackett-Burman experimental design. The results showed that 70% aqueous solution of acetone was the most efficient solvent for isolation of flavanones from mandarin peel. The main flavanones present in the obtained extracts were hesperidin (HES) and narirutin (NRT). The number of extraction stages influenced the yield of extraction, type of solvent influenced the hesperidin extraction efficiency and particle size of material influenced the narirutin extraction efficiency.

Flavanoni so pomembna skupina flavonoidov, ki so karakteristični za citruse. V naših raziskavah smo s konvencionalno ekstrakcijo izvedli izolacijo flavanonov iz lupin mandarin, pri čemer smo kot topilo uporabili vodo, aceton, etanol ter vodne raztopine acetona in etanola. Dobljene ekstrakte smo analizirali in določili sestavo in vsebnost flavanonov ter določili njihovo delovanje na stabilni DPPH radikal. V nadaljevanju smo s pomočjo Plackett-Burman-ovega eksperimentalnega načrta določili tudi vpliv ekstrakcijskih parametrov (velikost delcev, temperatura in čas ekstrakcije, razmerje material-topilo, število stopenj ekstrakcije ter vrsta topila) na učinkovitost ekstrakcije. Rezultati so pokazali, da je najbolj učinkovito topilo za izolacijo flavonoidov iz lupin mandarin 70% vodna raztopina acetona. Glavna flavanona, prisotna v ekstraktih, sta bila hesperidin in narirutin. Na izkoristek ekstrakcije vpliva število stopenj ekstrakcije, na učinkovitost ekstrakcije hesperidina vrsta topila, medtem ko ima velikost delcev materiala pomemben vpliv na učinkovitost ekstrakcije narirutina.