[Study on the enzyme extraction process of hypericin by pectinase].
Copyright policy )[Study on the enzyme extraction process of hypericin by pectinase].
To explore the best enzyme and optimal conditions for extracting Hypericin from Hypericum perforatum.Chose the best enzyme from Pectinase, Xylanase, Glucanase, beta-Glucanase and Enzyme (SPE-007A). The effeet of solid-liquid ratio enzyme dosage, PH, temperature and the extraction time were investigated by L9 (3(4)) orthogonal design using extraction rate and the content of Hypericin as assessment index.The best enzyme was Pectinase and the optimum extraction process was as follows: PH 4.6, enzyme dosage 1.5%, temperature 50 degrees C, extraction time 5 h, liquid-solid ratio 15 times.This method is efficient and stable. It could be used in the future research of Hypericum perforatum.
- Henan University China (People's Republic of)
Anthracenes, Analysis of Variance, Plants, Medicinal, Time Factors, Temperature, Reproducibility of Results, Dextranase, Hydrogen-Ion Concentration, Plant Components, Aerial, Polygalacturonase, Technology, Pharmaceutical, Perylene, Chromatography, High Pressure Liquid, Hypericum
Anthracenes, Analysis of Variance, Plants, Medicinal, Time Factors, Temperature, Reproducibility of Results, Dextranase, Hydrogen-Ion Concentration, Plant Components, Aerial, Polygalacturonase, Technology, Pharmaceutical, Perylene, Chromatography, High Pressure Liquid, Hypericum
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