Stoma is the main routeway for water and gas exchange in terrestrial plants, playing an important role on the global water and carbon cycles. Stomatal traits, including stomatal density, stomatal shape, stomatal size, and stomatal index, are the long term adaptation result of plants to environmental factors during evolution, and sensitive to the changes of environmental factors. This paper reviewed the last 30 years research advances in the relationships between stomatal traits and environmental factors (e.g., air CO2 concentration, temperature, water, and light, etc. ) and the main relevant investigation methods, and proposed the main directions of future research in stomatal traits in context of climate change.