The research, accomplished in the period 2004-2005, had as general objective to analyse teachers and students' perceptions of the biosafety teaching-learning process in secondary courses of the health area. The data were collected at Oswaldo Cruz Foundation courses in Rio de Janeiro. A total of 82 students and 12 teachers have participated on the study. It was a teorical-empirical research with qualitative basis and quantitative data that emerged throughout the working process. The data were analyzed in the multireferenciality context. This investigation is justified by discrepancy of the biosafety in relation to the work world and the school world and the current demands of the technical-scientific progress and of the own social evolution, in relation to the biosafety at the spaces of the health. The obtained results, pointed at a necessity of improvement on the teaching processes in biosafety in secondary courses of the health area. Then, the results may contribute to the inclusion of the biosafety in the health professional education.