Two experiments were conducted to determine the urinary purine derivative (PD) excretion rates and to analyze potential variations in PD excretions between yak, Tibetan indigenous cattle, and their crossbreds under similar ecological environment, and to develop prediction equations based on PD excretion for estimating rumen microbial protein supply. Three Tibetan castrated male yaks, and similar numbers of indigenous cattle and their crossbreds, were used in a fasting trial followed by a feeding trial, using three 3 x 3 Latin squares (1 for each breedtype), to measure responses of PD excretion to different feeding levels. The results showed that i) daily endogenous PD excretion for yak, indigenous cattle, and their crossbreds was 134, 163, and 138 micromol/kg of BW(0.75) (P = 0.38), respectively; and ii) crossbreds have greater PD excretion rate per unit digestible OM intake than indigenous cattle (P = 0.03). However, there were no significant differences between yaks and the crossbreds (P = 0.24) or between yaks and the indigenous cattle (P = 0.25). The proportion of allantoin to total PD in urine ranged from 0.83 to 0.88, with the crossbreds having greater values compared with the 2 parents (P = 0.03). Daily glomerular filtration rate, calculated using endogenous creatinine as an internal marker, for the above animals was 3.85, 4.23, and 3.61 L/kg of BW(0.75), respectively, in fasting trial (P = 0.59). The alpine animals may develop special regulating mechanisms in the kidney in terms of glomerular filtration rate and PD excretion, which would help the animals in adapting to the harsh environment of the Qinghai-Tibetan plateau.