In the State of Mato Grosso, 100% of the municipalities have records of autochthonous cases of American cutaneous leishmaniasis. The present study had the aim of investigating the geographical distribution of the Lutzomyia whitmani in the state. Mato Grosso has three distinct ecosystems: the savannah, the marshland and the area of the Amazon domain. Data on occurrences of Lutzomyia whitmani were obtained from reports on entomological surveys carried out by the Entomology Group of the National Health Foundation between 1996 and 2000 and by the Entomology Laboratory of the Mato Grosso State Health Department between 2001 and 2006. Entomological surveys were performed in 83 (59.7%) of the 139 municipalities of Mato Grosso. Lutzomyia whitmani was caught in 70 (84.3%) municipalities, with widespread distribution in all types of vegetation.